Specificity |
GenScript
MonoRab™ Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit specifically
recognizes His tags placed at C-terminal and internal regions of His-fusion
proteins. This product is not recommended for N-terminal His-fusion protein
detection. |
Host Species |
Rabbit |
Immunogen |
6x His synthetic peptide coupled to KLH |
Conjugate |
Unconjugated |
Working
concentrations for specific applications should be determined by the
investigator. The appropriate concentrations may be affected by secondary
antibody affinity, antigen concentration, the sensitivity of the method of
detection, temperature, the length of the incubations, and other factors. The
suitability of this antibody for applications other than those listed below has
not been determined. The following concentration ranges are recommended
starting points for this product. |
Application |
Recommended Usage |
ELISA |
0.05-0.2 μg/ml |
Western Blot |
0.1-0.2 μg/ml |
Immunoprecipitation (IP) |
2 μg/ml |
Immunocytochemistry/Immunofluorescence (ICC/IF) |
1 μg/ml |
Flow Cytometry |
1 μg/ml |
|
Form |
Lyophilized |
Storage Buffer |
Lyophilized
with PBS, pH 7.4, containing 0.02% sodium azide. |
Reconstitution |
Reconstitute
the lyophilized powder with deionized water (or equivalent) to a final
concentration of 0.5 mg/mL. |
Storage Instructions |
The
lyophilized product remains stable for up to 1 year at -20°C from date of
receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C, or for
up to 12 months at -20°C or below. Upon receiving, spin the vial down to place
the lyophilized antibody on the bottom of the vial. Reconstitute the antibody
as mentioned above. Prepare single use aliquots (no less than 10µl) and freeze
it immediately at -20oC. The frozen aliquots should be thawed once
with any remainder kept at 4oC. |
Purification |
Purified
by Protein A affinity chromatograph. |
Isotype |
Rabbit IgG |
Clonality |
Monoclonal |
Clone ID |
25B6E11 |
Note |
GenScript
can customize this product according to you requirement, including product size,
buffer components, etc. |
Figure 1. Sensitivity and specificity of MonoRab™ Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit (A01857) to different quantities of a recombinant His6-tagged protein was determined by Western blot. The primary antibody was A01857 at 0.2 μg/mL concentration. The secondary antibody was Goat anti-rabbit IgG (H&L) [IRDye800] (Licor,926-32211) at 0.125 μg/mL concentration.
Figure 2. Cross-reactivity of MonoRab™ Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit (A01857-40) was determined by Western blot. The primary antibodies were (A) A01857-40 at 1μg/mL concentration; and (B) Rabbit IgG (Negative control) at 1μg/mL concentration. The secondary antibody was Goat anti-Rabbit IgG (H&L) [IRDye800] (Licor,926-32211) at 0.125μg/mL concentration.
Figure 3. Cell lysates containing over-expressed His-tagged protein were used for immunoprecipitation using MonoRab™ Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit (A01857) and the result was analyzed by Western blot. The following were loaded:
Lane 1: Immunoprecipitation with MonoRab™ Anti-His antibody (4 μg)
Lane 2: Negative control – IP with Rabbit IgG (4 μg)
Lane 3: Positive control containing His-tagged protein
The primary antibody for Western blot detection was THE™ His tag antibody, mAb, Mouse (GenScript, A01857) at 0.2 μg/mL concentration. The secondary antibody was Goat anti-rabbit IgG (H&L) [IRDye800] (Licor,926-32211) at 0.125 μg/mL concentration.
Target Background |
The
rabbit immune system generates diverse antibodies and optimizes antibody affinity.
GenScript utilizes MonoRabTM technology to generate high affinity
and specific monoclonal rabbit antibodies. GenScript’s MonoRabTM
Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit is specific to six
histidine tag placed at the C-terminal and internal regions of fusion proteins.
This antibody can greatly improve the effectiveness of several different kinds
of immunoassays, helping researchers identify, detect, and purify polyhistidine
fusion proteins from bacteria, insect cells, and mammalian cells. |
Synonyms |
Rabbit
monoclonal to His tag |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.