Figure 1. Apelin-13-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/AGTRL1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with an AGTRL1 receptor agonist, Apelin-13.The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (10-fold dilution) of Apelin-13 (Mean ± SD, n = 2). The EC₅₀ of Apelin-13 on this cells was 0.19 μM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/AGTRL1/Gα15 Stable Cell Line

The apelin receptor AGTRL1 is Gi/Go-coupled GPCRs expressed in the heart, coronary artery, aorta, internal mammary artery, pulmonary artery and saphenous vein, it also expressed in lung, kidney and adrenal gland. In AGTRL1 receptor knockout mice baseline blood pressure is not changed compared to wild-type animals. Vasoconstrictor responses to angiotensin II however are significantly more pronounced in the knockout animals.

产品编号	M00245
规格	2 vials 定制报价
数量
价格	询价


联系凯发一触即发

Synonyms

APJ; APJ (apelin) receptor; APJ receptor; APJR; G protein-coupled receptor APJ; G-protein coupled receptor APJ; G-protein coupled receptor HG11; HG11; HG11 orphan receptor; angiotensin II receptor-like 1; angiotensin receptor-like 1; apelin receptor

产品概述

Applications

Functional assay for AGTRL1 receptor

产品简介

Storage	Liquid nitrogen immediately upon delivery
Species	Human

培养条件

Freeze Medium	45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium	Ham’s F-12 K (Kaighn’s), 10% FBS, 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen), 400 μg/ml G418 (Cat. #10131-035, Gibco)

图例

Figure 1. Apelin-13-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/AGTRL1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with an AGTRL1 receptor agonist, Apelin-13.The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (10-fold dilution) of Apelin-13 (Mean ± SD, n = 2). The EC₅₀ of Apelin-13 on this cells was 0.19 μM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

凯发一触即发

CHO-K1/AGTRL1/Gα15 Stable Cell Line

资源中心

技术支持

关于凯发一触即发