目录产品 » 稳定细胞系 » Human Recombinant CD64 Stable Cell Line

Figure 1: FACS analysis of cell surface expression of CD64 on CHO-K1/CD64 cells.
The CHO-K1/CD64 cells (blue) and the negative control CHO-K1 cells (red) were stained with PE Mouse anti-Human CD64 (Cat. No. 558592, BD Bioscience).

CHO-K1/CD64 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress high affinity human Fc fragment of IgG receptor Ia (FcγRI, CD64) on the cell surface, containing one alpha chain and two gamma chains. The surface expression of CD64 is validated by FACS analysis. This stable cell line product is designed for measuring the binding affinity and stability of the Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD64 during biologics research and development.

产品编号	M00588
规格	2 vials 定制报价
数量
价格	￥85000


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产品概述

Product Description

Applications

Cell-based binding assay; use as immunogen for antibody development

产品简介

Expressed Gene	Codon Optimized from NM_000566.3 (for FCGR1A); Codon Optimized from NM_004106.2 (for FCER1G); No expressed tags.
Host Cell	CHO-K1
Quantity	2 vials of frozen cells (>1×10⁶ per vial in 1 ml)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status	Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).

培养条件

Culture Properties	Adherent
Freeze Medium	95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium	Ham's F-12K (Kaighn's) (Cat. No.21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco)
Culture Medium	Ham's F-12K (Kaighn's) (Cat. No.21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco), 300 µg/ml Hygromycin B (Cat. No.10687010, Invitrogen), 8 μg/ml Puromycin (Cat. No.A11138-03, Gibco)

技术背景

Gene Synonyms

FCRI, CD64A, FCGR1, FCGR1A (for Fc Fragment of IgG receptor Ia); FCER1G, FCRG (for Fc fragment of IgE receptor Ig)

Background

CD64 (Cluster of Differentiation 64) is a type of integral membrane glycoprotein, also known as Fc-gamma receptor 1 (FcγRI). It is composed of two subunits- an alpha subunit encoded by FCGR1A (Fc Fragment of IgG Receptor Ia), and a gamma subunit encoded by FCER1G (Fc Fragment of IgE Receptor Ig). It binds monomeric IgG-type antibodies with high affinity. After binding IgG, CD64 interacts with an accessory chain known as the common γ chain (γ chain), which possesses an ITAM motif that is necessary for triggering cellular activation. While CD64 is constitutively found only on macrophages and monocytes, treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.

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