The CytoSinct™ CD3 Nanobeads, human are used for separating CD3+ cells from fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products or single cell suspension based on the surface expression of human CD3. The surface of Nanobeads are labeled with anti-human CD3 monoclonal antibody. To begin the isolation, Nanobeads are added to the cells. The CD3 antibodies preconjugated to the Nanobeads can bind the target cells expressing CD3 on cell surface. The cells/beads mixed suspension is loaded onto a CytoSinct^TM Column which is effectively magnetized by an external magnetic field from CytoSinct™ Magnet, or other compatible cell isolation columns and magnets. The Nanobeads-labeled CD3+ cells are retained within the column and enriched during the wash step when Isolation Buffer is used to flush out the CD3- cells. After removing magnetic field, the target CD3+ cells can be easily eluted from the column.