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Primate neural retina upregulates IL-6 and IL-10 in response to a herpes simplex vector suggesting the presence of a pro-/anti-inflammatory axis.

Exp Eye Res.. 2016-07; 
MM Sauter, CR Brandt.
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... Precautions were taken during virus preparation and all experiments to minimize endotoxin levels. High titer viral stocks were tested for endotoxin levels with the ToxinSensor Chromagenic LAL Endotoxin Assay Kit (Genscript, L00350, Piscataway, NJ). ...

摘要

Injection of herpes simplex virus vectors into the vitreous of primate eyes induces an acute, transient uveitis. The purpose of this study was to characterize innate immune responses of macaque neural retina tissue to the herpes simplex virus type 1-based gene delivery vector hrR3. PCR array analysis demonstrated the induction of the pro-inflammatory cytokine IL-6, as well as the anti-inflammatory cytokine IL-10, following hrR3 exposure. Secretion of IL-6 was detected by ELISA and cone photoreceptors and Muller cells were the predominant IL-6 positive cell types. RNA in situ hybridization confirmed that IL-6 was expressed in photoreceptor and Muller cells. The IL-10 positive cells in the inner nuclear layer wer... More

关键词

Gene therapy; HSV-1; IL-10; IL-6; Inflammation; Retina; Viral vectors