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An essential role of high-molecular-weight kininogen in endotoxemia

J. Exp. Med.. 2017; 
Aizhen Yang, * Zhanli Xie, * Bo Wang, * Robert W. Colman, Jihong Dai,, and Yi Wu,
Products/Services Used Details Operation
Rabbit polyclonal anti-HK D5 was generated by GenScript. The following peptides were commercially synthesized by GenScript: VSP PHTSMAPAQDEERDSGKEQGHTRRHDW (peptide 1), RDSGKEQGHTRRHDWGHEKQRKHNLGHGHK (peptide 2), GHEKQRKHNLGHGHKHERDQGHGH QRGHGL (peptide 3), HERDQGHGHQRGHGLGHG HEQQHGLGHGHK (peptide 4), GHGHEQQHGLGH GHKFKLDDDLEHQGGHVL (peptide 5), FKLDDD LEHQGGHVLDHGHKHKHGHGHGKH (peptide 6), DHGHKHKHGHGHGKHKNKGKKNGKHNGWK (peptide 7), and KNKGKKNGKHNGWKTEHLASSSED STTPS (peptide 8). Peptides 1–7 were derived from the D5 domain of HK, and peptide 8 was derived from the N terminus of the D6 domain. The protein purity, size, and identity were verified by Coomassie brilliant blue staining and immunoblotting using a mouse anti-His antibody (catalog no. A00186; GenScript) with the use of a positive control Multiple-tag (catalog no. M0101; GenScript) and protein markers (catalog no. MM0908; GenScript).The hybridoma producing mAb C11C1 was generated as previously described (Song et al., 2004; Khan et al., 2010) and purified by GenScript. An anti-DHG15 antibody was produced by GenScript, using a peptide (DHGHKHKHG HGHGKHC conjugated with KLH) HPLC purified to >96% purity as immunogen.

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