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N-terminal methionine excision of proteins creates tertiary destabilizing N-degrons of the Arg/N-end rule pathway.

J. Biol. Chem.. 2019-03; 
NguyenKha The,KimJeong-Mok,ParkSang-Eun,HwangCheol-
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摘要

All organisms begin protein synthesis with methionine (Met). The resulting initiator Met of nascent proteins is irreversibly processed by Met aminopeptidases (MetAPs). N-terminal (Nt) Met excision (NME) is an evolutionarily conserved and essential process operating on up to two-thirds of proteins. However, the universal function of NME remains largely unknown. MetAPs have a well-known processing preference for Nt-Met with Ala, Ser, Gly, Thr, Cys, Pro, or Val at position 2, but using CHX-chase assays to assess protein degradation in yeast cells, as well as protein-binding and RT-qPCR assays, we demonstrate here that NME also occurs on nascent proteins bearing Met-Asn or Met-Gln at their N ter... More

关键词

N-end rule,N-terminal acetylation,N-terminal amidase,N-terminal arginylation,N-terminal methionine excision,acetylation,proteasome,protein degradation,ubiquitin,ubiquitin li