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Functions of duplicated glucosinolate sulfatases in the development and host adaptation of Plutella xylostella

Insect Biochem Mol Biol. 2020; 
Chen W, Dong Y, Ahmed Saqib HS, Vasseur L, Zhou W, Zheng L, Lai Y, Ma X, Lin L, Xu X, Bai J, He W, You M.
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摘要

Evolutionary adaptations of herbivorous insects are often dictated by the necessity to withstand a corresponding evolutionary innovation in host plant defense. Glucosinolate sulfatase (GSS) enzyme activity is considered a central adaptation strategy in Plutella xylostella against glucosinolates (GS)-myrosinase defense system in the Brassicales. The high functional versatility of sulfatases suggests that they may perform other vital roles in the process of growth and development. Here, we used a CRISPR/Cas9 system to generate stable homozygous single/double mutant lines of gss1 or/and gss2 with no predicted off-target effects, to analyze the functions of the pair of duplicated genes in the development and host a... More

关键词

CRISPR/Cas9; adaptation; diamondback moth; gene duplication; homozygous mutant lines; sulfatases