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An RNA degradation machine sculpted by Ro autoantigen and noncoding RNA.

Cell.. 2013-03;  153(1):166-77
Chen X, Taylor DW, Fowler CC, Galan JE, Wang HW, Wolin SL. Department of Cell Biology, Yale School of Medicine, New Haven, CT 06510, USA.
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...To form the RNP-antibody complex, we incubated 100 nM of the GraFix-prepared RNP and 50 nM of monoclonal anti-His (A00186, <b>Genscript</b>)...

摘要

SummaryMany bacteria contain an ortholog of the Ro autoantigen, a ring-shaped protein that binds noncoding RNAs (ncRNAs) called Y RNAs. In the only studied bacterium, Deinococcus radiodurans, the Ro ortholog Rsr functions in heat-stress-induced ribosomal RNA (rRNA) maturation and starvation-induced rRNA decay. However, the mechanism by which this conserved protein and its associated ncRNAs act has been obscure. We report that Rsr and the exoribonuclease polynucleotide phosphorylase (PNPase) form an RNA degradation machine that is scaffolded by Y RNA. Single-particle electron microscopy, followed by docking of atomic models into the reconstruction, suggests that Rsr channels single-stranded RNA into the PNP... More

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