BLI (Biolayer interferometry) binding affinity measurements of MonoRab™ Anti-FMC63 Antibody (24B2), mAb, Rabbit to FMC63 scFv.
FMC63 scFv-his captured on Anti-His (HIS) Probes can bind MonoRab™ Anti-FMC63 Antibody (24B2), mAb, Rabbit (GenScript, A02327) with a dissociation constant (KD) of 7.81E-10M.

Flow cytometric analysis of MonoRab™ Anti-FMC63 Antibody (24B2), mAb, Rabbit specificity for FMC63 scFv.
The specificity of the MonoRab™ Anti-FMC63 Antibody (24B2), mAb, Rabbit was evaluated using Raji cells as the experimental system. In the first group, Raji cells were incubated with a 1:50 dilution of MonoRab™ Anti-FMC63 Antibody (24B2), mAb, Rabbit (GenScript, A02327) followed by staining with a PE conjugated anti-rabbit IgG antibody, showing a negative signal (blue line). In the second group, Raji cells were incubated with FMC63 scFv, followed by staining with the same concentration of MonoRab™ Anti-FMC63 Antibody (24B2), mAb, Rabbit (GenScript, A02327) and PE conjugated anti-rabbit IgG antibody, resulting in a positive signal (red line) .

MonoRab™ Anti-FMC63 Antibody (24B2), mAb, Rabbit binds to FMC63 scFv in ELISA assays.
Recombinant FMC63 scFv was coated at a concentration of 1 μg/mL, followed by the addition of MonoRab™ Anti-FMC63 Antibody (24B2), mAb, Rabbit (GenScript, A02327) at serially diluted concentrations starting from 1 μg/mL. Detection was performed using Mouse Anti-Rabbit IgG Fc Antibody (14H9H10)[HRP], mAb (GenScript, A01856) diluted at 1:20,000.

The detection of PBMCs with or without FMC63 CAR-T cells using iFluor 647 conjugated MonoRab™ Anti-FMC63 Antibody (24B2), mAb, Rabbit (GenScript, A02327) demonstrated minimal nonspecific binding to PBMCs and specific recognition of FMC63-expressing CARs.