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Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons.

J Vis Exp.. 2014-09; 
X Zhao, Y Zhou, AM Weissmiller, ML Pearn, W C Mobley, C Wu. Department of Neurosciences, University of California, San Diego.
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摘要

Axonal transport of BDNF, a neurotrophic factor, is critical for the survival and function of several neuronal populations. Some degenerative disorders are marked by disruption of axonal structure and function. We demonstrated the techniques used to examine live trafficking of QD-BDNF in microfluidic chambers using primary neurons.

关键词

Neuroscience; Issue 91; live imaging; brain-derived neurotrophic factor (BDNF); quantum dot; trafficking; axonal retrograde transport; microfluidic chamber