Comprehensive structural optimization of chimeric
antigen receptors (CARs) is the key to their successful
performance both in vitro and in vivo. In order to compare various CAR designs in an unified format, we took
advantage of a lentiviral platform, where all CAR modules can be easily shuffled and tested for functionality.
This platform was used to delineate the effects of various
spacer regions on the function of a PSCA-specific CAR
in the context of a human NK cell line, YT. We show
that three CAR designs (IgG1-, CD8a-, and spacerless)
perform similarly in vitro regardless of the length of the
spacer region.
Comprehensive structural optimization of chimeric
antigen receptors (CARs) is the key to their successful
performance both in vitro and in vivo. In order to compare various CAR designs in an unified format, we took
advantage of a lentiviral platform, where all CAR modules can be easily shuffled and tested for functionality.
This platform was used to delineate the effects of various
spacer regions on the function of a PSCA-specific CAR
in the context of a human NK cell line, YT. We show
that three CAR designs (IgG1-, CD8a-, and spacerless)
perform similarly in vitro regardless of the length of the
spacer region.
