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TOP1α regulates expression by coupling histone modification and transcription machinery.

Development. 2019-03; 
ZhongPeiqiao, LiJiaojiao, LuoLinjie, ZhaoZhong, TianZha
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… of Science and Technology of China, Huangshan Road 443, Hefei 230027, China. Contact … 5-8 times with pull-down solution, separated on an SDS-PAGE gel, and detected by anti-GST antibody (GenScript; A00866-100, Lot: 13D000626). Development ? Accepted manuscript …

摘要

The key steps of transcription are coupled with the opening of the DNA helical structure and establishment of active chromatin to facilitate the movement of the transcription machinery. Type I topoisomerases cleave one DNA strand and relax the supercoiled structure of transcribed templates. How topoisomerase-mediated DNA topological changes promote transcription and establish a permissive histone modification for transcription elongation is largely unknown. Here, we show that TOPOISOMERASE 1α in plants regulates transcription by coupling histone modification and transcription machinery. We demonstrate that TOP1α directly interacts with the methyltransferase SDG8 to establish high levels of H3K36 methylatio... More

关键词

Arabidopsis,FLOWERING LOCUS C,H3K36 methyltransferase,RNA polymerase II,Topoisomerase I,Transcription elonga