凯发一触即发

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Fbxl4 Serves as a Clock Output Molecule that Regulates Sleep through Promotion of Rhythmic Degradation of the GABA Receptor.

Curr. Biol.. 2017; 
Li Qian,Li Yi,Wang Xiao,Qi Junxia,Jin Xi,Tong Huawei,Zhou Zikai,Zhang Zi Chao,Han Ju
Products/Services Used Details Operation
Recombinant Proteins Antiserum was purified by the His6-Nrv3 fusion protein. All three antibodies were generated by GenScript (Nanjing, China). Other antibodies used in this study were: HA-tag (3724; Cell Signaling Technology, Danvers, MA, USA); PDF (C7; Developmental Studies Hybridoma Bank, Iowa City, IA, USA); Tubulin (T6199; Sigma, St. Louis, MO, USA); Actin (R15006MC4; Yurogen, Worcester, MA, USA); Myc-tag (Sigma, M4439); Ubi (ab7254; Abcam, Cambridge, UK); and His-tag (A00186; GenScript, Piscataway, NJ, USA). Get A Quote

摘要

The timing of sleep is tightly governed by the circadian clock, which contains a negative transcriptional feedback loop and synchronizes the physiology and behavior of most animals to daily environmental oscillations. However, how the circadian clock determines the timing of sleep is largely unclear. In vertebrates and invertebrates, the status of sleep and wakefulness is modulated by the electrical activity of pacemaker neurons that are circadian regulated and suppressed by inhibitory GABAergic inputs. Here, we showed that Drosophila GABA receptors undergo rhythmic degradation in arousal-promoting large ventral lateral neurons (lLNvs) and their expression level in lLNvs displays a daily oscillation. We... More

关键词

Drosophila,E3 ligase,Fbx14,GABA(A) receptor,circadian clock,degradation,pacemaker neurons,s