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Cloning, Purification, and Characterization of Recombinant Human Extracellular Superoxide Dismutase in SF9 Insect Cells.

Mol. Cells. 2016; 
ShresthaPravesh,YunJi-Hye,KimWoo Taek,KimTae-Yoon,LeeWeo
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Biochemicals … G1 Affinity resin (Cat. No. L00432). FLAG-tagged hEC-SOD was then purified following the protocol provided by GenScript. Purified hEC-SOD was simultaneously concentrated to 0.3 mM (9 mg/ml). The final buffer was exchanged Get A Quote

摘要

A balance between production and degradation of reactive oxygen species (ROS) is critical for maintaining cellular homeostasis. Increased levels of ROS during oxidative stress are associated with disease conditions. Antioxidant enzymes, such as extracellular superoxide dismutase (EC-SOD), in the extracellular matrix (ECM) neutralize the toxicity of superoxide. Recent studies have emphasized the importance of EC-SOD in protecting the brain, lungs, and other tissues from oxidative stress. Therefore, EC-SOD would be an excellent therapeutic drug for treatment of diseases caused by oxidative stress. We cloned both the full length (residues 1-240) and truncated (residues 19-240) forms of human EC-SOD (hEC-... More

关键词

LC-MS/MS,extracellular matrix (ECM),human extracellular superoxide (hEC-SOD),reactive oxygen species (ROS),sodiumdiethyldithiocarba