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Impact of dynamin 2 on adenovirus nuclear entry.

Virology. 2019-03; 
LeeJi Sun,IsmailAshrafali M,LeeJeong Yoon,ZhouXiaohong,MaterneEmma C,ChodoshJames,Rajaiya
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Proteins, Expression, Isolation and Analysis All cells tested negative for endotoxin contamination by ToxinSensor chromogenic LAL (Limulus amebocyte lysate) endotoxin assay kit (GenScript, Piscataway, NJ, cat. no. L00350), Get A Quote

摘要

The large GTPase dynamin 2 controls both endosomal fission and microtubule acetylation. Here we report that dynamin 2 alters microtubules and regulates the trafficking of human adenovirus type 37. Dynamin 2 knockdown by siRNA in infected cells resulted in accumulation of acetylated tubulin, repositioning of microtubule organizing centers (MTOCs) closer to cell nuclei, increased virus in the cytosol (with a compensatory decrease in endosomal virus), reduced proinflammatory cytokine induction, and increased binding of virus to the nucleoporin, Nup358. These events led to increased viral DNA nuclear entry and viral replication. Overexpression of dynamin 2 generated opposite effects. Therefore, dynamin ... More

关键词

Adenovirus,Cytokines,Dynamin 2,Microtubule organizing center,Microtubules,Nucleoporin,Viral traffic