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RCC1-dependent activation of Ran accelerates cell cycle and DNA repair, inhibiting DNA damage-induced cell senescence.

Mol. Biol. Cell. 2016; 
CekanPavol,HasegawaKeisuke,PanYu,TubmanEmily,OddeDavid,ChenJin-Qiu,HerrmannMichelle A,KumarSheetal,Kalab
Products/Services Used Details Operation
Proteins, Expression, Isolation and Analysis Abcam (Cambridge, MA; RCC1, ab109379; TPX2 ab32785), Cell Signaling Technology (Danvers, MA; RanBP1, 8780; Chk2, 3440), Epitomics (Burlingame, CA; pS10H3, 1173-1), Developmental Studies Hybridoma Bank (Iowa City, IA; tubulin E7, E7), Millipore (γH2AX(Ser-193), 05-636), and GenScript (Piscataway, NJ; β-actin, A00702). Get A Quote

摘要

The coordination of cell cycle progression with the repair of DNA damage supports the genomic integrity of dividing cells. The function of many factors involved in DNA damage response (DDR) and the cell cycle depends on their Ran GTPase-regulated nuclear-cytoplasmic transport (NCT). The loading of Ran with GTP, which is mediated by RCC1, the guanine nucleotide exchange factor for Ran, is critical for NCT activity. However, the role of RCC1 or Ran⋅GTP in promoting cell proliferation or DDR is not clear. We show that RCC1 overexpression in normal cells increased cellular Ran⋅GTP levels and accelerated the cell cycle and DNA damage repair. As a result, normal cells overexpressing RCC1 evaded DNA dama... More

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