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The RXLR Effector Avrblb2 Modulates Plant Immunity by Interfering With Ca Signaling Pathway.

Front Plant Sci. 2019-01; 
NaveedZunaira Afzal,BibiShaheen,AliGul
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Proteins, Expression, Isolation and Analysis … Co-immunoprecipitation assays were done using the Glutathion MagBeads (L00327, GenScript) according to the manufacturer's protocol with modifications as follows. One hundred μL of Glutathione MagBeads were washed 3 × with TBST … Get A Quote

摘要

In plants, subcellular fluctuations in Ca ion concentration are among the earliest responses to biotic and abiotic stresses. Calmodulin, which is a ubiquitous Ca ion sensor in eukaryotes, plays a major role in translating these Ca signatures to cellular responses by interacting with numerous proteins located in plasma membranes, cytoplasm, organelles and nuclei. In this report, we show that one of the RXLR effector, Avrblb2, interacts with calmodulin at the plasma membrane of the plant cells. Using deletion and single amino acid mutagenesis, we found that calmodulin binds to the effector domain of Avrblb2. In addition, we show that most known homologs of Avrblb2 in three different species ... More

关键词

AVRblb2 effector,RXLR effector,Rpi-blb2,calcium signaling,calmodulin (CAM),plant-Phytophthora interact