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Enhancing humoral immunity via sustained-release implantable microneedle patch vaccination.

Proc. Natl. Acad. Sci. U.S.A.. 2019; 
BoopathyArchana V,MandalAnasuya,KulpDaniel W,MenisSergey,BennettNitasha R,WatkinsHannah C,WangWade,MartinJacob T,ThaiNikki T,HeYanpu,SchiefWilliam R,HammondPaula T,IrvineDarre
Products/Services Used Details Operation
Catalog Antibody Briefly, Maxisorp 96-well plates (Nunc) were coated with rabbit anti-6xHis antibody (Genscript, A00174).... Maxisorp 96-well plates (Nunc) were coated with rabbit anti-6xHis tag antibody (Genscript, A00174) or anti-STII (Novus Biologicals, NBP2-41073) at 2 μg/mL in PBS for 4 h at 25 °C and blocked with PBS containing 5% nonfat milk, 10% goat serum, 1% BSA, 1% FBS, and 0. Get A Quote

摘要

Sustained exposure of lymphoid tissues to vaccine antigens promotes humoral immunity, but traditional bolus immunizations lead to rapid antigen clearance. We describe a technology to tailor vaccine kinetics in a needle-free platform translatable to human immunization. Solid pyramidal microneedle (MN) arrays were fabricated with silk fibroin protein tips encapsulating a stabilized HIV envelope trimer immunogen and adjuvant, supported on a dissolving polymer base. Upon brief skin application, vaccine-loaded silk tips are implanted in the epidermis/upper dermis where they release vaccine over a time period determined by the crystallinity of the silk matrix. Following MN immunization in mice, Env trimer was... More

关键词

antibody response,microneedles,vaccine deli