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Xrs2 and Tel1 Independently Contribute to MR-Mediated DNA Tethering and Replisome Stability.

Cell Rep. 2018; 
Oh Julyun,Lee So Jung,Rothstein Rodney,Symington Lorrai
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摘要

The yeast Mre11-Rad50-Xrs2 (MRX) complex has structural, signaling, and catalytic functions in the response to DNA damage. Xrs2, the eukaryotic-specific component of the complex, is required for nuclear import of Mre11 and Rad50 and to recruit the Tel1 kinase to damage sites. We show that nuclear-localized MR complex (Mre11-NLS) catalyzes homology-dependent repair without Xrs2, but MR cannot activate Tel1, and it fails to tether DSBs, resulting in sensitivity to genotoxins, replisome instability, and increased gross chromosome rearrangements (GCRs). Fusing the Tel1 interaction domain from Xrs2 to Mre11-NLS is sufficient to restore telomere elongation and Tel1 signaling to Xrs2-deficient cells... More

关键词

DNA repair,DNA replication,Mre11,Rad50,Tel1,Xrs2,genome stabi