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The synergistic effect of combination temozolomide and chloroquine treatment is dependent on autophagy formation and p53 status in glioma cells.

Cancer Lett.. 2015; 
Lee Seung Woo,Kim Hyun-Kyung,Lee Na-Hyeon,Yi Hee-Yeon,Kim Hong-Sug,Hong Sung Hee,Hong Yong-Kil,Joe Youn
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Catalog Antibody The membrane was incubated with the designated primary antibodies [Beclin 1 (Cell Signaling, #3738, Beverly, MA, USA), phospho-p53 (Cell signaling, #9284), p53 (Santa Cruz, sc-126, Dallas, TX, USA), V5-tag (Genscript, A01724, Piscataway, NJ, USA) and β-actin (Sigma, A5441)], and then with horseradish peroxidase-conjugated secondary antibodies. Get A Quote

摘要

Temozolomide (TMZ) is an alkylating agent used for the treatment of glioblastoma. The late autophagy inhibitor chloroquine (CQ) inhibits glioblastoma tumors in a p53-independent and p53-dependent manner. We addressed a possible beneficial effect of combination treatment with TMZ and CQ by examining the molecular and cellular mechanism of co-treatment. Combination treatment of U87 cell (wild type p53) with TMZ and CQ synergistically reduced cell proliferation and enhanced apoptosis, with increased sub-G1 hypodiploid cells and caspase activation. This effect was abolished by a pan-caspase inhibitor, Z-VAD-FMK. TMZ induced autophagy, and the addition of CQ further increased autophagic vacuoles. Inhibition of... More

关键词

Autophagy,Chloroquine,Glioma,Temozolomide