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Histone Modifications Regulate Chromatin Compartmentalization by Contributing to a Phase Separation Mechanism

Mol Cell.. 2019; 
Wang L1, Gao Y1, Zheng X2, Liu C3, Dong S1, Li R1, Zhang G1, Wei Y1, Qu H2, Li Y1, Allis CD4, Li G3, Li H5, Li P6.
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摘要

Eukaryotic chromosomes contain compartments of various functions, which are marked by and enriched with specific histone modifications. However, the molecular mechanisms by which these histone marks function in chromosome compartmentalization are poorly understood. Constitutive heterochromatin is a largely silent chromosome compartment characterized in part by H3K9me2 and 3. Here, we show that heterochromatin protein 1 (HP1), an H3K9me2 and 3 "reader," interacts with SUV39H1, an H3K9me2 and 3 "writer," and with TRIM28, an abundant HP1 scaffolding protein, to form complexes with increased multivalent engagement of H3K9me2 and 3-modified chromatin. H3K9me2 and 3-marked nucleosomal arrays and associated complexes ... More

关键词

H3K9me3; HP1; SUV39H1; TRIM28; chromatin compartmentalization; heterochromatin; histone modifications; nuclear extract; nucleosomal arrays; phase separation