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| Catalog Antibody | Methods Materials The following is a list of antibodies used for western blot- ting and their dilutions used in 3% milk: GAPDH, Millipore Cat#AB2302 (1:30000); GluA1, Abcam Cat#ab76321 (1:1000); NR1, Zymed Cat#32–500 (1:1000); NR2B, Mil- lipore Cat#AB1557P (1:1000); Rabbit anti-Gβ1, a gift from Dr Willardson PMID:15485848 (1:6000); synaptophysin, Assay Biotech Cat#C0333 (1:1000); PSD-95, Cell Signal- ing Cat# 3450 (1:1000); GABAARα1, Neuromab Cat#75- 136 (1:1000); c-Myc, GenScript Cat#A00172 (1:1000); ELFN2, Sigma Cat#HPA000781 (1:1000); mGluR7, Upstate Cat#07-239 (1:2000); mGluR8 GeneTex Cat#GTX82530 (1:500); mGluR4, EMD Millipore Cat#AB15097 (1:2000); mGluR2, Santa Cruz Cat#sc- 271655 (1:500); mGluR5, Millipore Cat#06451.... Lysate combinations were incubated with protein G beads conjugated to c-Myc antibody (GenScript Cat#A00172) for ~1 h at 4 °C and washed three times with centrifugation and fresh lysis buffer. | Get A Quote |
The functional characterization of the GPCR interactome has predominantly focused on intracellular binding partners; however, the recent emergence of transsynaptic GPCR complexes represents an additional dimension to GPCR function that has previously been unaccounted for in drug discovery. Here, we characterize ELFN2 as a novel postsynaptic adhesion molecule with a distinct expression pattern throughout the brain and a selective binding with group III metabotropic glutamate receptors (mGluRs) in trans. Using a transcellular GPCR signaling platform, we report that ELFN2 critically alters group III mGluR secondary messenger signaling by directly altering G protein coupling kinetics and efficacy. Loss of ELFN2 in ... More
