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SHROOM3-FYN Interaction Regulates Nephrin Phosphorylation and Affects Albuminuria in Allografts.

J Am Soc Nephrol. 2018; 
Wei C, Banu K, Garzon F, Basgen JM, Philippe N, Yi Z, Liu R, Choudhuri J, Fribourg M, Liu T, Cumpelik A, Wong J, Khan M, Das B, Keung K, Salem F, Campbell KN, Kaufman L, Cravedi P, Zhang W, O'Connell PJ, He JC, Murphy B, Menon MC.
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Mutagenesis Services 4 Site-specific mutagenesis replacing -CCC/-CCA (Proline) with -GCC/-GCA (Alanine) by proprietary recombi- nase (GenScript Inc.... Lysates were subjected to immunoblot analysis using polyclonal rabbit anti-SHROOM3 (#SAB3500818; Sigma), anti-V5 tag antibody (A01724; GenScript Inc. Get A Quote

摘要

We previously showed that the presence of a CKD-associated locus in SHROOM3 in a donor kidney results in increased expression of SHROOM3 (an F-actin-binding protein important for epithelial morphogenesis, via rho-kinase [ROCK] binding); this facilitates TGF-b signaling and allograft fibrosis. However, other evidence suggests Shroom3 may have a protective role in glomerular development.,We used human data, Shroom3 knockdown podocytes, and inducible shRNA-mediated knockdown mice to study the role of Shroom3 in adult glomeruli.,Expression data from the Nephroseq database showed glomerular and nonglomerular SHROOM3 had opposing associations with renal function in CKD biopsy samples. In human allografts, homozygosit... More

关键词

Cell Signaling; gene expression; podocyte; renal transplantation