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Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity.

Proc Natl Acad Sci U S A. 2018; 
Wang Q,, Li Y,, Ishikawa K,, Kosami KI, Uno K, Nagawa S,,, Tan L, Du J, Shimamoto K, Kawano Y,.
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Catalog Antibody The beads were then washed five times for 5 min each time with pull-down buffer, and the bound proteins were eluted with 100 μl 2× SDS loading buffer and subjected to immunoblot assay with anti- SUMO (A01693; GenScript), anti-GST (sc-138; Santa Cruz Biotechnology), and anti-MBP (E8032S; New England Biolabs) antibodies. Get A Quote

摘要

Resistance (R) genes encode intracellular nucleotide-binding/leucine-rich repeat-containing (NLR) family proteins that serve as critical plant immune receptors to induce effector-triggered immunity (ETI). NLR proteins possess a tripartite domain architecture consisting of an N-terminal variable region, a central nucleotide-binding domain, and a C-terminal leucine-rich repeat. N-terminal coiled-coil (CC) or Toll-interleukin 1 receptor (TIR) domains of R proteins appear to serve as platforms to trigger immune responses, because overexpression of the CC or TIR domain of some R proteins is sufficient to induce an immune response. Because direct downstream signaling molecules of R proteins remain obscure, the molecu... More

关键词

GEF; R protein; effector-triggered immunity; rice; small GTPase