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Activation of delta-opioid receptor contributes to the antinociceptive effect of oxycodone in mice.

Pharmacol Res. 2016; 
Yang PP, Yeh GC, Yeh TK, Xi J, Loh HH, Law PY, Tao PL.
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Gene Synthesis Briefly, the CHO-K1/OPRM1/G␣15 cells co-expressing the human MOR (in pEntry) and G␣15 (M00304, GenScript) or HEK293/OPRD1/G16 (transfection of HEK293 cell line hDOR (in pcDNA3) with G16 lentivirus) expressing the human DOR and G16 were cultured in CORNING® black with clear flat bottom 96-well assay plate with 200 ␮l of HAM’s/F12 medium contain- ing 10% fetal calf serum, pen/strep, 1 x Glutamax (Gibco), zeocin (200 ␮g/ml) and hygromycin (100 ␮g/ml) (CHO-K1/OPRM1/G␣15) or Advanced DMEM medium (InvitrogenTM ) containing 5% fetal calf ␮g/ml) under serum, pen/strp, 1xGlutamax (Gibco) and G418 (200 ␮l of 5% CO2 atmosphere until confluence. Get A Quote

摘要

Oxycodone has been used clinically for over 90 years. While it is known that it exhibits low affinity for the multiple opioid receptors, whether its pharmacological activities are due to oxycodone activation of the opioid receptor type or due to its active metabolite (oxymorphone) that exhibits high affinity for the mu-opioid receptors remains unresolved. Ross and Smith (1997) reported the antinociceptive effects of oxycodone (171nmol, i.c.v.) are induced by putative kappa-opioid receptors in SD rat while others have reported oxycodone activities are due to activation of mu- and/or delta-opioid receptors. In this study, using male mu-opioid receptor knock-out (MOR-KO) mice, we examined whether delta-opioid rece... More

关键词

Antinociceptive; DAMGO (PubChem CID: 5462471); Etorphine (PubChem CID: 443407); Morphine (PubChem CID: 528826); Naloxone hydrochloride (PubChem CID: 5464092); Naltrindole hydrochloride (PubChem CID: 24840086); Opioid receptors; Oxycodone; Oxycodone hydrochloride (PubChem CID: 5462350); Oxymorphone; Oxymorphone (PubChem CID: 5284604)