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T cell epitope of arginine kinase with CpG co-encapsulated nanoparticles attenuates a shrimp allergen-induced Th2-bias food allergy.

Biosci Biotechnol Biochem. 2019; 
Gao Q,, Hong J,, Xiao X, Cao H, Yuan R, Liu Z,, Chen T.
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Cellular Analysis … mmol/L imidazole, pH 8.0). The endotoxin was replaced using an ion-exchange column and ToxinEraserTM Endotoxin Removal Kit (L00338, Genscript, China). Immunogenicity identification of AKp To verify the immunogenicity … Get A Quote

摘要

T cell peptide-based immunotherapy (PIT) is an appealing therapeutic strategy for modulating allergic responses without IgE cross-linking. We propose a novel PIT that combines a T-cell epitope of the shrimp allergen arginine kinase (AKp) with TLR9 agonist CpG-ODN in nanoparticles (CpG-AKp NPs) to attenuate a shrimp allergen-induced food allergy. Treatment with CpG-AKp NPs demonstrated the attenuation of anaphylaxis responses such as the reduced incidence of diarrhea and hypothermia, lower levels of specific IgE and the induction of IgG2a in serum. Th2 cytokines were suppressed and higher Th1 cytokines were detected in the splenocyte culture supernatants. Treatment of CpG-AKp NPs also enhanced the protein expres... More

关键词

CpG-ODN; Shrimp allergy; T epitopes; arginine kinase; immunotherapy