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Biochemical And Functional Characterization Of Recombinant Fungal Immunomodulatory Proteins (Rfips).

Int Immunopharmacol.. 2013-02; 
Bastiaan-Net S, Chanput W, Hertz A, Zwittink RD, Mes JJ, Wichers HJ. Food and Biobased Research, Wageningen University and Research Centre, Bornse Weilanden 9, Wageningen, The Netherlands.
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摘要

In this study two novel FIPs have been identified and characterized. The first is FIP-nha, identified in the ascomycete Nectria haematococca, and as such, FIP-nha would be the first FIP to be identified outside the order of Basidiomycota. The second is LZ-9, an LZ-8 like protein identified in Ganoderma lucidum. Recombinant FIP proteins were produced in Pichia pastoris and purified using His-affinity magnetic beads. The bioactive characteristics of FIP-nha and LZ-9 were compared to two other well-known FIP proteins, LZ-8 from G. lucidum and FIP-fve from Flammulina velutipes, which were produced and purified using the same method. The produced recombinant FIPs (rFIPs): rLZ-8, rLZ-9, rFIP-fve and rFIP-nha were inv... More

关键词

Fungal immunomodulatory proteins (FIPs); Hemagglutination; Caco-2 cells; Transepithelial electrical resistance (TEER); THP-1 macrophages; Immunomodulation