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Coronavirus endoribonuclease targets viral polyuridine sequences to evade activating host sensors

PNAS. 2020-03; 
Matthew Hackbart, Xufang Deng, and Susan C. Baker
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Catalog Antibody One microgram of RNA was mixed with dsRNA antibody (K1; Scicons) or mouse anti-Beta actin (A00702-40; Genscript), RNA binding buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Nonidet P-40, and 20 U/mL Ribolock [EO0381; Thermofisher]). Get A Quote

摘要

Coronaviruses (CoVs) are positive-sense RNA viruses that can emerge from endemic reservoirs and infect zoonotically, causing significant morbidity and mortality. CoVs encode an endoribonuclease designated EndoU that facilitates evasion of host pattern recognition receptor MDA5, but the target of EndoU activity was not known. Here, we report that EndoU cleaves the 5′-polyuridines from negative-sense viral RNA, termed PUN RNA, which is the product of polyA-templated RNA synthesis. Using a virus containing an EndoU catalytic-inactive mutation, we detected a higher abundance of PUN RNA in the cytoplasm compared to wild-type−infected cells. Furthermore, we found that transfecting PUN RNA into cells stimulates a ... More

关键词

EndoU; coronavirus; endoribonuclease; interferon; nsp15