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Plasmodium falciparum histidine rich protein HRPII inhibits the anti-inflammatory function of antithrombin

J Thromb Haemost. 2019; 
Dinarvand P, Yang L, Biswas I, Giri H, Rezaie AR, .
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Gene Synthesis … The endotoxin level of HRPII following passage through the column was essentially undetectable (<6 x10-4 ng/μg HRPII) as analyzed by an Endotoxin Assay Kit (#L00350, GenScript, Piscataway, NJ). After verifying its homogeneity … Get A Quote

摘要

It has been reported that histidine-rich protein II (HRPII), secreted by the malaria parasite, Plasmodium falciparum (Pf), inhibits the heparin-dependent anticoagulant activity of antithrombin (AT) in vitro and in plasma-based assay systems.,The objective of this study was to test the hypothesis that HRPII may also interact with the AT-binding vascular glycosaminoglycans (GAGs), thereby inhibiting the anti-inflammatory signaling function of the serpin.,We expressed HRPII in bacteria, purified it to homogeneity and studied its effect on endothelial cell signaling in the absence and presence of AT employing established signaling assays.,We demonstrate that a low concentration of HRPII potently disrupts the barrie... More

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