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Bispecific Antibody Fragment Targeting APP and Inducing α-Site Cleavage Restores Neuronal Health in an Alzheimer's Mouse Model

Mol Neurobiol. 2019; 
He P, Xin W, Schulz P, Sierks MR.
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Proteins, Expression, Isolation and Analysis … The brain tissue was homogenized in a homogenization buffer. Multiple tag (cata- log: M0101, GenScript, Piscataway, NJ), a designed fusion protein and molecular weight of ~ 40 kDa on SDS-PAGE, contains FLAG sequence DYKDDDDK … Get A Quote

摘要

The amyloid β (Aβ) peptide, correlated with development of Alzheimer's disease (AD), is produced by sequential proteolytic cleavage of the amyloid precursor protein (APP) by β- and γ-secretases. Alternative proteolytic cleavage of APP by α-secretase prevents formation of Aβ peptide and produces a neuroprotective protein, a soluble fragment of APPα (sAPPα). We previously generated a single-chain variable domain antibody fragment (scFv) that binds APP at the β-secretase cleavage site and blocks cleavage of APP (iBsec1), and a second scFv which has been engineered to have α-secretase-like activity that increases α-secretase cleavage of APP (Asec1a) and showed that a bispecific antibody (Diab) combining ... More

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