Genscript, cat. no. RP01002) Glass beads, 0.5 mm (Soda lime, BioSpec, ...">

凯发一触即发

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Genome-wide measurement of protein-DNA binding dynamics using competition ChIP.

Nat Protoc.. 2013-06;  8(7 ):1337-53
Lickwar CR, Mueller F, Lieb JD. Department of Biology, Carolina Center for the Genome Sciences, Curriculum in Genetics and Molecular Biology and Lineberger Comprehensive Cancer Center, Chapel Hill, North Carolina, USA.
Products/Services Used Details Operation

摘要

Competition chromatin immunoprecipitation (competition ChIP) enables experimenters to measure protein-DNA dynamics at a single locus or across the entire genome, depending on the detection method. Competition ChIP relies on a cell containing two copies of a single DNA-associated factor, with each copy of the factor differentially epitope tagged. One of the copies is expressed constitutively and the second is induced as a competitor. The ratio of isoforms associated with discrete genomic locations is detected by ChIP-on-chip (ChIP-chip) or ChIP-sequencing (ChIP-seq). The rate at which the resident isoform of the protein is replaced by the competitor at each binding location enables the calculation of residence t... More

关键词