| Products/Services Used | Details | Operation |
|---|---|---|
| Cellular Analysis | … PK activity was detected using a PK Assay Kit (Jiancheng Technology Co Ltd, Nanjing, China) … Precast gels of 10% (M00664, Genscript, USA) were used to analyze total content and S-nitrosylated content of GP, PFK and PK … | Get A Quote |
The activity, expression and S-nitrosylation of glycogen phosphorylase (GP), phosphofructokinase (PFK) and pyruvate kinase (PK) was compared between pale, soft and exudative (PSE) and red, firm and non-exudative (RFN) pork. The nitric oxide synthase (NOS) activity of RFN pork was higher than PSE pork (P < 0.05). Glycogen and lactic acid content were significantly different between PSE and RFN samples at 1 h postmortem (P < 0.05). Compared to PSE pork, RFN pork had lower activities and higher S-nitrosylation levels of GP, PFK and PK (P < 0.05). Moreover, GP expression in RFN pork was lower (P < 0.05) while no significant differences of PFK and PK expression were observed between these two groups. These ... More
