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The effect of local delivery of adiponectin from biodegradable microsphere-scaffold composites on new bone formation in adiponectin knockout mice

J Mater Chem B. 2016-06-01; 
Dan Li, Yuan Guo, Hui Lu, Ren Wang, Hong-Cheng Hu, Song-He Lu, Xue-Fen Li, Zi-Chen Li, Yu-Wei Wu, Zhi-Hui Tang
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Recombinant Proteins … His-gAPN was then purified using a GE Pharmacia AKTA Purifier 10 (Ramsey, MN, USA) followed by endotoxin removal using a ToxinEraser™ Endotoxin Removal Kit L00338 (GenScript, Piscataway, NJ, USA) and isolation using Zeba Spin Desalting Columns, 7k MWCO, 10 … Get A Quote

摘要

Adiponectin (APN) is the most abundant adipocyte-secreted adipokine; it regulates energy homeostasis and exerts well-characterized insulin-sensitizing properties. Previous studies have verified that globular adiponectin (gAPN) is also involved in bone metabolism, although observations have been controversial. The purpose of the current study is to use an APN-knockout (APN-KO) mouse model to evaluate the local delivery of gAPN to new bone formation. Using chitosan microspheres (CMs), we found that following an initial burst at 1 week, the release behavior of gAPN from the scaffold was sustained in a linear manner for the first 4 weeks, followed by a slower, more stable release from week 5 onwards. Interestingly,... More

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