凯发一触即发

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Molecular cloning and functional characterization of Physcomitrella patens UBC13-UEV1 genes required for Lys63-linked polyubiquitination

Plant Sci. 2020; 
Huiping Guo, Linxiao Wang, Ruoyang Hu, Yikun He, Wei Xiao
Products/Services Used Details Operation
Catalog Antibody … analysis. The blots were probed with anti-His 6 (MBL, D291-3) and anti-GST (GenScript, A00865-100) primary antibodies, and chemiluminescence signals were detected by autoradiography. 2.6. Ub conjugation reaction. In vitro … Get A Quote

摘要

Ubc13 and Ubc/E2 variant (Uev) form a stable heterodimer to mediate Lys63-linked polyubiquitination. Unicellular eukaryotic genomes often contain single UBC13 and UEV gene; however, multiple homologs were found in higher plants. As initial land plants, Physcomitrella patens occupies a key evolutionary position between green algae and higher plants. In this study, we report the identification and functional characterization of two UBC13 and three UEV1 genes from P. patens. Both PpUbc13s form heterodimers with PpUev1B or PpUev1C, which catalyze Lys63-linked polyubiquitination in vitro and functionally complement the yeast ubc13 mms2 null mutant from killing by DNA-damaging agents. In contrast, PpUev1A is unable t... More

关键词

DNA-damage tolerance, K63-linked polyubiquitination, Physcomitrella patens, Ubc13, Uev1