凯发一触即发

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Efficient process development for high-level production, purification, formulation, and characterization of recombinant mecasermin in Escherichia coli

Biotechnol Appl Biochem. 2020; 
Mohammad Reza Mofid, Valiollah Babaeipour, Sevda Jafari, Leila Haddad, Sharareh Moghim, Jalaledin Ghanavi
Products/Services Used Details Operation
Endotoxin Detection & Removal System … horseshoe crabs. In this study, LAL Endotoxin Assay Kit (Genscript, L00350C) was used to measure endotoxin … mM Na Acetat, 100mM NaCl, pH 5.4. CD-spectra were measured The Toxin Sensor Chromogenic LAL Endotoxin Assay Kit (Genscript, L00350C) was used … Get A Quote

摘要

Overproduction of recombinant mecasermin was achieved by investigation of effect of three factors, temperature, inducer amount, and culture media, at three levels according to the Taguchi statistical design in Escherichia coli in a bench-scale bioreactor. In optimal conditions (induction temperature 28 °C, terrific broth with glucose (TB+G) medium, with 0.1 mM IPTG as inducer) 0.84 g/L mecasermin with expression levels of 38% of total protein and 4.13 g/L final dry cell biomass was produced, that is one of the highest values of recombinant protein has been reported in the batch system. The cell disruption was done by lysozyme pretreatment with sonication to the efficient purification of mecasermin. The isolat... More

关键词

Mecasermin, characterization, fermentation, purification, rhIGF-1