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Exosomal miR-1255b-5p targets human telomerase reverse transcriptase in colorectal cancer cells to suppress epithelial-to-mesenchymal transition

Mol Oncol. 2020; 
Xue Zhang, Jian Bai, Hang Yin, Long Long, Zhewen Zheng, Qingqing Wang, Fengxia Chen, Xiaoyan Yu, Yunfeng Zhou
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Proteins, Expression, Isolation and Analysis … protein. For the GST pull-down assay, GST-hTERT was cooled on ice and mixed with 50 μL of GST MagBeads (L00327, GenScript, Piscataway, NJ) and 800 μL of 1% Triton X-100 in PBS for 1 h at 4°C. The beads were then … Get A Quote

摘要

Cancer cells undergo epithelial-to-mesenchymal transition (EMT) in response to hypoxia. Exosomes produced in tumor microenvironments carry microRNAs (miRNAs) that affect proliferation, metastasis, and EMT. Hypoxic regulation of EMT is associated with telomerase content and stability, but the underlying mechanisms remain unclear. We identified a targeting relationship between tumor-suppressing miR-1255b-5p and human telomerase reverse transcriptase (hTERT) via clinical screening of serum samples in colorectal cancer (CRC) patients. EMT suppression via exosomal miR-1255b-5p delivery was investigated by assessing hTERT expression, Wnt/β-catenin signaling, and telomerase activity. We revealed that hypoxia directly... More

关键词

epithelia-to-mesenchymal transition, exosome, hTERT, microRNA, microenvironment