GenScript, A00172; China), P16 (Abcam, ab50282; Cambridge, UK), GAPDH (ProteinTech, 60004-1; China), or green fluorescent protein (GFP) (ProteinTech, 50430-2-AP; China) was applied at 1:5,000 and 1:1,000 dilutions.?...">

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The p16-Specific Reactivation and Inhibition of Cell Migration Through Demethylation of CpG Islands by Engineered Transcription Factors.

Hum Gene Ther.. 2012-10;  23(10):1071-1081
Zhang B, Xiang S, Zhong Q, Yin Y, Gu L, Deng D. Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Division of Cancer Aetiology, Peking University Cancer Hospital/Institute, Beijing, 100142, China.
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摘要

Methylation of CpG islands inactivates transcription of tumor suppressor genes including p16 (CDKN2A). Inhibitors of DNA methylation and histone deacylation are recognized as useful cancer therapeutic chemicals through reactivation of the expression of methylated genes. However, these inhibitors are not target gene-specific, so that they lead to serious side effects as regular cytotoxic chemotherapy agents. To explore the feasibility of methylated gene-specific reactivation by artificial transcription factors, we engineered a set of Sp1-like seven-finger zinc-finger proteins (7ZFPs) targeted to a 21-bp sequence of the p16 promoter and found that these 7ZFPs could bind specifically to the target p16 promoter pro... More

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