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SETD7 regulates chondrocyte differentiation and glycolysis via the Hippo signaling pathway and HIF‑1α

Int J Mol Med. 2021-10; 
Maoquan Li, Jinqiu Ning, Jiwei Wang, Qiqian Yan, Ke Zhao, Xiaoshi Jia
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Proteins, Expression, Isolation and Analysis … Proteins were separated by sodium dodecyl sulfate-poly-acrylamide 10% gel electrophoresis (GenScript; cat. no. M00665) and transferred … Next, 20 µl protein A/G-agarose beads (Santa Cruz Biotechnology, Inc.) was added and the samples were rocked for 3 h at 4C. The … Get A Quote

摘要

Chondrocytes are well adapted to hypoxia and produce more functional extracellular matrix in low oxygen environments . In our previous study, methyltransferase SET domain containing (SETD)7 regulated chondrocyte activity in hypoxic conditions. However, the precise association between SETD7 and chondrocyte differentiation under low oxygen partial pressure remains unclear. The association between SETD7 and chondrocyte differentiation was studied by silencing SETD7 in chondrocytes . The results showed that the silencing of SETD7 in ATDC5 cells inhibited the Hippo signaling pathway, decreased Yes‑associated protein (YAP) phosphorylation and increased the levels of YAP and hypoxia inducible factor‑1α (HIF‑1α... More

关键词

Hippo, SET domain containing 7, Yes‑associated protein, differentiation, energy metabolism, hypoxia inducible factor‑1α