凯发一触即发

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Dnj1 Promotes Virulence in by Maintaining Robust Endoplasmic Reticulum Homeostasis Under Temperature Stress

Front Microbiol. 2021-09; 
Linda C Horianopoulos, Christopher W J Lee, Guanggan Hu, Mélissa Caza, James W Kronstad
Products/Services Used Details Operation
Gene Synthesis … Genes encoding ER chaperones are upregulated under stress conditions related to virulence including thermotolerance in C. neoformans (Yang et … 10000 anti-HA monoclonal antibody (2-2.2.14, ThermoFisher) and THE™ beta Actin antibody (A00702, GenScript, Piscataway, NJ … Get A Quote

摘要

The capacity of opportunistic fungal pathogens such as to cause disease is dependent on their ability to overcome an onslaught of stresses including elevated temperature under mammalian host conditions. Protein chaperones and co-chaperones play key roles in thermotolerance. In this study, we characterized the role of the endoplasmic reticulum (ER) J-domain containing co-chaperone, Dnj1, in the virulence of . A strain expressing a Dnj1-GFP fusion protein was used to confirm localization to the ER, and a deletion mutant was shown to be hypersensitive to the ER stress caused by tunicamycin (TM) or 4μ8C. Dnj1 and another ER chaperone, calnexin were found to coordinately maintain ER homeostasis and contribute to ... More

关键词

JDPs, co-chaperones, endoplasmic reticulum, fungal pathogenesis, heat shock, proteostasis