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GDSL lipase occluded stomatal pore 1 is required for wax biosynthesis and stomatal cuticular ledge formation

New Phytol. 2020-07; 
Jing Tang, Xianpeng Yang, Chuanlei Xiao, Jiaying Li, Yongqiang Chen, Ruiying Li, Shipeng Li, Shiyou Lü, Honghong Hu
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Proteins, Expression, Isolation and Analysis … Zhang et al., 2017). The secreted recombinant proteins were purified on High Affinity Ni- NTA Resin (L00250; GenScript Biotech Corp., Piscataway, NJ, USA) according to the manufacturer's instructions. The concentra- tions … Get A Quote

摘要

The plant leaf surface is coated with a waterproof cuticle layer. Cuticle facing the stomatal pore surface needs to be sculpted to form outer cuticular ledge (OCL) after stomatal maturation for efficient gas exchange. Here, we characterized the roles of Arabidopsis GDSL lipase, Occlusion of Stomatal Pore 1 (OSP1), in wax biosynthesis and stomatal OCL formation. OSP1 mutation results in significant reduction in leaf wax synthesis and occlusion of stomata, leading to increased epidermal permeability, decreased transpiration rate, and enhanced drought tolerance. We demonstrated that OSP1 activity is critical for its role in wax biosynthesis and stomatal function. In vitro enzymatic assays demonstrated that OSP1 po... More

关键词

OSP1, Arabidopsis, drought tolerance, epidermal permeability, stomatal outer cuticular ledge, thioesterase activity, water loss, wax biosynthesis