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Histone demethylase PHF8 promotes cell growth and metastasis of non-small-cell lung cancer through activating Wnt/β-catenin signaling pathway

Histol Histopathol. 2021-06; 
Yan Hu, Hanshuo Mu, Ying Yang
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Catalog Antibody … Then the lysates were immunoprecipitated with control IgG (A01008, GenScript, USA), anti-PHF8 (ab36068, Abcam), anti-H3K27me2 (ab24684, Abcam), anti-H3K9me1 (ab8896, Abcam), anti-H3K9me2 (ab1220, Abcam) and anti- H4K20me1 (ab9051, Abcam) … Get A Quote

摘要

PHD finger protein 8 (PHF8), serving as a histone demethylase, is upregulated in some types of malignant tumors. The role of PHF8 in non-small-cancer lung carcinoma (NSCLC) remains unclear. This study aims to verify the effect of PHF8 in NSCLC and its molecular mechanism. We collected 20 cases of fresh NSCLC and adjacent lung tissues to assess differential expressions of PHF8 by reverse transcription-quantitative PCR (RT-qPCR). Western blot was employed to examine protein levels of PHF8, Wnt1, β-catenin and epithelial-mesenchymal transition (EMT) related proteins. Chromatin immunoprecipitation assays were executed to confirm the regulatory mechanism of PHF8 and Wnt1. Cell Counting Kit-8 assays and Transwell as... More

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