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Transcriptomic and Phenotypic Analysis of CRISPR/Cas9-Mediated Knockout in Zebrafish

Genes (Basel). 2022-08; 
Qianqian Yan, Wenhao Li, Xiaoting Gong, Ruiqin Hu, Liangbiao Chen
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GenCrispr Cas9 Genome Editing … The MAXIscriptTMT7 in Vitro Transcription Kit (AM1314) was purchased from Ambion (USA) and the GenCrispr NLS-Cas-NLS Nuclease (Z03389-100) was purchased from GenScript (… Get A Quote

摘要

As a subtype of kainite receptors (KARs), GluK2 plays a role in the perception of cold in the periphery sensory neuron. However, the molecular mechanism for on the cold stress in fish has not been reported. In this article, real-time PCR assays showed that was highly expressed in the brain and eyes of adult zebrafish. To study the functions of , gene knockout was carried out using the CRISPR/Cas9 system. According to RNA-seq analysis, we selected the differentially expressed genes (DEGs) that had significant differences in at least three tissues of the liver, gill, intestine, skin, brain, and eyes. Gene Ontology (GO) enrichment analysis revealed that , , , , , , , , , , , , , , and were significantly enriche... More

关键词

RNA-seq, behavior analysis, cold stress, gluk2, knockout