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Recapitulating early human development with 8C-like cells

Cell Rep. 2022-06; 
Xiu Yu, Shiqi Liang, Manqi Chen, Hanwen Yu, Ruiqi Li, Yuliang Qu, Xuhui Kong, Ruirui Guo, Rongyan Zheng, Zsuzsanna Izsvák, Chuanbo Sun, Mingzhu Yang, Jichang Wang
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Proteins, Expression, Isolation and Analysis … Protein samples were separated by 4–20% SurePAGE precast gel (Genscript, cat#M00656) and then transferred to nitrocellulose membranes (Millipore, catalog. no. HATF00010). … Get A Quote

摘要

In human embryos, major zygotic genome activation (ZGA) initiates at the eight-cell (8C) stage. Abnormal ZGA leads to developmental defects and even contributes to the failure of human blastocyst formation or implantation. An in vitro cell model mimicking human 8C blastomeres would be invaluable to understanding the mechanisms regulating key biological events during early human development. Using the non-canonical promoter of LEUTX that putatively regulates human ZGA, we developed an 8C::mCherry reporter, which specifically marks the 8C state, to isolate rare 8C-like cells (8CLCs) from human preimplantation epiblast-like stem cells. The 8CLCs express a panel of human ZGA genes and have a unique transcriptome r... More

关键词

8CLC, CP: Stem cell research, DUX4, LEUTX, ZSCAN4, blastoid, preimplantation development, zygotic genome activation