凯发一触即发

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

A prion-like domain of TFEB mediates the co-aggregation of TFEB and mHTT

Autophagy. 2022-06; 
Junsheng Yang, Huilin Xu, Chaoyue Zhang, Xiaotong Yang, Weijie Cai, Xiaoli Chen
Products/Services Used Details Operation
Proteins, Expression, Isolation and Analysis … The normal functions of ALP take multiple steps and require the cooperation of … on 4-12% gradient SDS-PAGE gels (GenScript, M00654) and transferred to polyvinylidene difluoride … Get A Quote

摘要

The aggregation of mutant HTT (huntingtin; mHTT) is a hallmark of Huntington disease (HD). mHTT aggregates interact and sequester dozens of proteins and affect diverse key cellular functions. Here we report that TFEB (transcription factor EB), a master regulator of lysosome biogenesis and autophagy, is yet another protein that co-aggregates with mHTT. We also found the mHTT-TFEB co-aggregation is mediated by a prion-like domain (PrLD) near the N terminus of TFEB. Our findings point out a possible limitation for therapeutic strategies targeting TFEB to clear mHTT, and also provided a possible explanation for controversies that TFEB overexpression lowered soluble mHTT in some HD models but failed to reduce mHTT a... More

关键词

Aggregate, HD, TFEB, mHTT, prion-like domain