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Zipper head mechanism of telomere synthesis by human telomerase

Cell Res. 2021-11; 
Futang Wan, Yongbo Ding, Yuebin Zhang, Zhenfang Wu, Shaobai Li, Lin Yang, Xiangyu Yan, Pengfei Lan, Guohui Li, Jian Wu, Ming Lei
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Proteins, Expression, Isolation and Analysis The eluent was then incubated with anti-Flag resin (GenScript Biotech, L00425) overnight. Get A Quote

摘要

Telomerase, a multi-subunit ribonucleoprotein complex, is a unique reverse transcriptase that catalyzes the processive addition of a repeat sequence to extend the telomere end using a short fragment of its own RNA component as the template. Despite recent structural characterizations of human and Tetrahymena telomerase, it is still a mystery how telomerase repeatedly uses its RNA template to synthesize telomeric DNA. Here, we report the cryo-EM structure of human telomerase holoenzyme bound with telomeric DNA at resolutions of 3.5 Å and 3.9 Å for the catalytic core and biogenesis module, respectively. The structure reveals that a leucine residue Leu980 in telomerase reverse transcriptase (TERT) catalytic ... More

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