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A Rapid Assay for SARS-CoV-2 Neutralizing Antibodies That Is Insensitive to Antiretroviral Drugs

J Immunol. 2021-06; 
Deli Huang, Jenny Tuyet Tran, Linghang Peng, Linlin Yang, Raymond T Suhandynata, Melissa A Hoffman, Fangzhu Zhao, Ge Song, Wan-Ting He, Oliver Limbo, Sean Callaghan, Elise Landais, Raiees Andrabi, Devin Sok, Joseph G Jardine, Dennis R Burton, James E Voss, Robert L Fitzgerald, David Nemazee
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Catalog Antibody Plates were coated with rabbit anti-His mAb (A00174; GenScript) diluted in PBS at 2 µg/ml overnight at 4°C Get A Quote

摘要

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike pseudotyped virus (PSV) assays are widely used to measure neutralization titers of sera and of isolated neutralizing Abs (nAbs). PSV neutralization assays are safer than live virus neutralization assays and do not require access to biosafety level 3 laboratories. However, many PSV assays are nevertheless somewhat challenging and require at least 2 d to carry out. In this study, we report a rapid (<30 min), sensitive, cell-free, off-the-shelf, and accurate assay for receptor binding domain nAb detection. Our proximity-based luciferase assay takes advantage of the fact that the most potent SARS-CoV-2 nAbs function by blocking the binding between S... More

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