| Specificity |
This antibody can be used to detect GST-fusion proteins in almost expression systems including bacterial expression system, mammalian expression system and other systems. |
| Host Species |
Rabbit |
| Immunogen |
Recombinant Glutathione-S-Transferase from Schistosoma japonicum |
| Species Reactivity |
Schistosoma japonicum |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.05-0.5 µg/ml |
| Western Blot |
0.1-1.0 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
Supplied in PBS, pH 7.4 with 0.02% sodium azide |
| Reconstitution |
Reconstitute the lyophilized antibody with deionized water (or equivalent) to the final concentration of 0.5 mg/ml. |
| Storage Instructions |
The product remains stable up to 1 year at -20 ℃ from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 ℃ or for up to 12 months at -20 ℃ or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Affinity chromatography |
| Isotype |
Rabbit IgG |
| Clonality |
Polyclonal |
| Clone ID |
Not applicable |
Western blot analysis of immunoprecipitates from GST fusion protein lysates. GST-tagged protein was immunoprecipitated with
Rabbit Anti-GST-Tag Polyclonal Antibody (GenScript, A00097) and specificity was confirmed by immunprecipitation with rabbit IgG.
Western blot was performed using Rabbit Anti- GST-Tag Polyclonal Antibody (GenScript, A00097).
Western blot analysis of GST fusion protein using 1 μg/ml Rabbit Anti-GST-tag Polyclonal Antibody (GenScript, A00097)
The result was developed with One-Step WesternTM Basic Kit (Rabbit) (GenScript, L00204)
| Target Background |
GenScript Rabbit Anti-GST-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. The immunogen used is a recombinant version of glutathione-S-transferase from Schistosoma japonicum. Glutathione-S-transferase is an epitope tag in generating fusion proteins. Antibodies specific to fusion proteins are useful in monitoring protein expression in Western Blots as well as immunoaffinity purification of proteins expressed in E. coli. The glutathione-S-transferases are a family of enzymes that catalyze the addition of the tripeptide glutathione to endogenous and xenobiotic substrates which have electrophilic functional groups. They play an important role in the detoxication and metabolism of many xenobiotic and endobiotic compounds. The glutathione adducts produced have increased solubility in water and are subsequently enzymatically degraded to mercapturates and excreted. |
| Synonyms |
Rabbit Anti-GST-tag pAb |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
