| Specificity |
GenScript Rabbit Anti-V5-tag Polyclonal Antibody specifically recognizes V5 tagged fusion proteins. |
| Host Species |
Rabbit |
| Immunogen |
V5 epitope tag peptide GKPIPNPLLGLDST conjugated to KLH |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigator.Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.05-0.2 µg/ml |
| Western Blot |
0.1-1.0 µg/ml |
| Western Blot |
1:100-1:1,000 |
|
| Form |
Lyophilized |
| Storage Buffer |
Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
| Storage Instructions |
The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Affinity chromatography |
| Isotype |
Rabbit IgG |
| Clonality |
Polyclonal |
| Clone ID |
Not applicable |
Western blot analysis of V5-tagged fusion protein using V5-tag Antibody, pAb, Rabbit (GenScript, A00623, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD
Western blot analysis of V5-tagged fusion protein using V5-tag Antibody, pAb, Rabbit (GenScript, A00623, 1 µg/ml)
The signal was developed with One-Step WesternTM Basic Kit (Rabbit) (GenScript, L00204)
Predicted Size: 52 kD
Observed Size: 52 kD
Western blot analysis of V5 tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-V5-tag Polyclonal Antibody (GenScript, A00623, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
| Target Background |
The V5 epitope tag is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope. |
| Synonyms |
Rabbit Anti-V5-tag pAb;Anti-V5-tag |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
