| Specificity |
This Antibody recognizes Protein C tagged fusion proteins. |
| Host Species |
Rabbit |
| Immunogen |
Protein C epitope tag peptide CEDQVDPRLIDGK conjugated to KLH |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.05-0.2 µg/ml |
| Western Blot |
0.1-1.0 µg/ml |
| Western Blot |
1:100-1:1,000 |
|
| Form |
Lyophilized |
| Storage Buffer |
Lyophilized with PBS, pH 7.4, containing 0.02%sodium azide |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
| Storage Instructions |
The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Affinity chromatography |
| Isotype |
Rabbit IgG |
| Clonality |
Polyclonal |
| Clone ID |
Not applicable |
Loading: Protein C tag fusion protein expressed in E. coli cell lysate
Antibody: Rabbit Anti-Protein C (HPC4)-tag Polyclonal Antibody (GenScript, A00637)
The result was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)
Western blot analysis of Protein C tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-Protein C-tag Polyclonal Antibody (GenScript, A00637, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
| Target Background |
Protein C is a Vitamin K-dependent plasma zymogen that is activated by proteolytic cleavage of the thrombin-thrombomodulin complex to form an anticoagulant enzyme.Protein C Tag is a 12 amino acid sequence EDQVDPRLIDGK, which encodes residues 6 through 17 of the heavy chain of the protein.Protein C Tag Antibody is a useful tool in analysis of Protein C fusion proteins.Genscript Rabbit Anti-Protein C (HPC4)-tag antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. |
| Synonyms |
Rabbit Anti-Protein C (HPC4)-tag pAb; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
